LOCUS       40924_24069        4551 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Cloning vector pHA1011 DNA, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4551)
  AUTHORS   Kasai Y, Oshima K, Ikeda F, Abe J, Yoshimitsu Y, Harayama S.
  TITLE     Construction of self-cloning system in a unicellular green algae, 
            Pseudochoricystis ellipsoidea
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4551)
  AUTHORS   Kasai Y, Oshima K, Ikeda F, Abe J, Yoshimitsu Y, Harayama S.
  TITLE     Direct Submission
  JOURNAL   Submitted (22-DEC-2014) Contact:Yuki Kasai Chuo University, 
            Biological Science; 1-13-27 Kasuga, Bunkyo-ku, Tokyo 112-8551, Japan
REFERENCE   3  (bases 1 to 4551)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 4551)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (22-DEC-2014) Contact:Yuki Kasai Chuo University, Biological 
            Science; 1-13-27 Kasuga, Bunkyo-ku, Tokyo 112-8551, Japan"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..4551
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        24..128
                     /label=AmpR promoter
     CDS             129..986
                     /label=AmpR
                     /note="beta-lactamase"
     rep_origin      1160..1748
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     protein_bind    2036..2057
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     promoter        2072..2102
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    2110..2126
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     2134..2150
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        2171..2189
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     regulatory      2238..3031
                     /note="includes promoter region of Pseudochoricystis
                     ellipsoidea nuclear gene RBCS for ribulose bisphosphate 
                     carboxylase/oxygenase small subunit"
                     /regulatory_class="promoter"
     misc_feature    3032..3310
                     /note="includes intron 1 from Pseudochoricystis ellipsoidea
                     RBCS gene"
     misc_feature    3311..3366
                     /label=multiple cloning site of pBluescript SK(+)
                     /note="multiple cloning site of pBluescript SK(+)"
     regulatory      3367..3878
                     /note="includes terminator region of Pseudochoricystis 
                     ellipsoidea RBCS gene"
                     /regulatory_class="terminator"
     promoter        complement(3908..3926)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(3936..3952)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     rep_origin      complement(4093..4548)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
ORIGIN
        1 gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt
       61 caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa
      121 ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt
      181 gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt
      241 tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt
      301 ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg
      361 tattatcccg tattgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga
      421 atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa
      481 gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga
      541 caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa
      601 ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca
      661 ccacgatgcc tgtagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta
      721 ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac
      781 ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc
      841 gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag
      901 ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga
      961 taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt
     1021 agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata
     1081 atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag
     1141 aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa
     1201 caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt
     1261 ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc
     1321 cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa
     1381 tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa
     1441 gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc
     1501 ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa
     1561 gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa
     1621 caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg
     1681 ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc
     1741 tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg
     1801 ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg
     1861 agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg
     1921 aagcggaaga gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat
     1981 gcagctggca cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaatg
     2041 tgagttagct cactcattag gcaccccagg ctttacactt tatgcttccg gctcgtatgt
     2101 tgtgtggaat tgtgagcgga taacaatttc acacaggaaa cagctatgac catgattacg
     2161 ccaagcgcgc aattaaccct cactaaaggg aacaaaagct ggagctccac cgcggtggcg
     2221 gccgctctag aactagtatg ccctcaatcg gccgatttca tgcatgaaga agaagatgat
     2281 gattacacat cattccagcc atctagggct gccatcagta attgcatgac ccgcaacttc
     2341 ccagtcagtc aaatgcacaa tagaagcata gttgcatggg aatcaaccca gcagcctccc
     2401 gcagagctag cctaggtacg tgtacgcagc cacataatca tcatgaatga ttctgcacgg
     2461 tgctatgata gcaacagata ctcgccagtg cgcacgcaag caatctcatc acaccactgt
     2521 ggtcaggcaa gcacgatata gatgtcaaat gacagctcca ttgggagtgc aagtctacta
     2581 gagtgcaagc ctactagtag taggaaggtc cagcgcatgc caaaactcct gctaaggggt
     2641 tgcccaagcc ccttggctgg ccaaaagtcc ttaatatttt gctaggtaaa ggggatcttc
     2701 ccggaggtgg tgttgatgaa catttgtaca ccagcatccg ggtccttttg gactctgaaa
     2761 acgtgagcga gcagcgactg cttgcagctt ccaaacgaaa ggtctggaag cgctggagtg
     2821 gtgagtcagg ggtgactcag caaacaccaa ggacacacgt tttcacccta ccctcagaac
     2881 ccttgaaata ggggtcgatt tccctctaac ttaagtggtt atccacaaac tcacgacgat
     2941 aagagccagc gccagcacca tttcgttcct cattttcagc tgaaagcaaa gcctttgcat
     3001 acttatcgct ctcagattac aatcgcaatg ggtacgcagc tttctcactc cctcaccgcg
     3061 tgtggttcac tcaggtttct ggctaaggaa agtctgatgt gactttcctt agacgttaaa
     3121 gcagaatcac cccatgcttt aagcagtttt atgtgtcttg cccttcatgt gaagggtctg
     3181 tactgttgca gagtgctctc ttgtgttctt gagtgttctt gtctccagtg tgcccattaa
     3241 ctttatccat gtcttatgtg tccaggactg cgccaaagaa actgacgctg cgctgttttg
     3301 ctttgtgcag ccatgggatc ccccgggctg caggaattcg atatcaagct tatcgatacc
     3361 gtcgacaggt tctaagcaat gacctgggct gtacatcatt agcaaactga atctgcagct
     3421 ttctaggctc ttgctgttcc taggaacttg gggttcaaga ctgggttttt gctgtggttc
     3481 ctgactgcag accatcactg tctgggttgt cgtgtctaat gtccataggt gtgagcacat
     3541 gctccacttc aattcctact gctgggtaac tggcgctgtc tgagggactt ctgagaagaa
     3601 tgccgagaag aacttgagcg atttgtaagg ggatattttg ggtctgtacc aaaggcccag
     3661 actgggcttc cagtgcatcg cacatatgca tctgcatgtc cattcataca atcaacctgt
     3721 tgactgcatg acccagatca caacctcctt gagttggaag tagcttcttc tacagataga
     3781 aaatggtgca tgtcagctga gggttgcagg cgtttaaaag tgcatcaagt ttgcagaaca
     3841 catggtgatt catgactgca tggaaacaga ccagcacact cgaggggggg cccggtaccc
     3901 aattcgccct atagtgagtc gtattacgcg cgctcactgg ccgtcgtttt acaacgtcgt
     3961 gactgggaaa accctggcgt tacccaactt aatcgccttg cagcacatcc ccctttcgcc
     4021 agctggcgta atagcgaaga ggcccgcacc gatcgccctt cccaacagtt gcgcagcctg
     4081 aatggcgaat ggaaattgta agcgttaata ttttgttaaa attcgcgtta aatttttgtt
     4141 aaatcagctc attttttaac caataggccg aaatcggcaa aatcccttat aaatcaaaag
     4201 aatagaccga gatagggttg agtgttgttc cagtttggaa caagagtcca ctattaaaga
     4261 acgtggactc caacgtcaaa gggcgaaaaa ccgtctatca gggcgatggc ccactacgtg
     4321 aaccatcacc ctaatcaagt tttttggggt cgaggtgccg taaagcacta aatcggaacc
     4381 ctaaagggag cccccgattt agagcttgac ggggaaagcc ggcgaacgtg gcgagaaagg
     4441 aagggaagaa agcgaaagga gcgggcgcta gggcgctggc aagtgtagcg gtcacgctgc
     4501 gcgtaaccac cacacccgcc gcgcttaatg cgccgctaca gggcgcgtca g
//
